Kuze kube manje, ukuhlaziywa kwe-PCR kuthathwa njengenye yezindlela ezinokwethenjelwa kakhulu zokuthola izifo ezihlukahlukene ezithathelwanayo. Ngaphezu kwalokho, le ndlela iba lula kakhulu. Ngenxa yezinga eliphakeme lokwaziswa, kungenzeka ukuthi ukuthola imiphumela yamanga kukhishwe.
Indlela yokuhlaziywa
Ngesikhathi sokuhlaziywa, impahla yokuhlolwa ifakwa esicelweni esikhethekile. Engeza ama-enzyme ahilelekile ekwakheni izinto eziphathekayo zofuzo. Khona-ke kukhona ukukopishwa okuphindaphindiwe kwe-DNA noma i-RNA ye-agent ye-causative yesifo. Kusukela kumjikelezo kuya komjikelezo, inani lamakhophi e-DNA landa kunani lapho kulula ukubona khona i-pathogen.
Ukuhlolwa kwegazi usebenzisa indlela ye-PCR kuvame ukusetshenziselwa ukwenza umtholampilo ukuveza imbangela yesifo esithathelwanayo. Kungenzeka futhi ukutadisha umchamo, i-smear emqaleni kanye nezinye izinto eziphilayo. Kwabesifazane, ukuhlaziywa kwe-PCR, izicathulo ezivela ezithombeni zobulili, isisindo esivela ku-urethra , isingeniso somlomo wesibeletho sisetshenziswa. Kubalulekile ukwazi ukuthi ungalungiselela kanjani ukuhlaziywa kwe-PCR kwabesifazane, ukuze umphumela unokwethenjelwa ngangokunokwenzeka. Into esemqoka ukugcina imithetho elandelayo:
- ukugwema izenzo zobulili zingakapheli izinsuku ezintathu ngaphambi kokulethwa kwezivivinyo;
- amabhaktheriya amaningi angakwazi "ukugeza" ngomchamo, ngakho ungavumeli ngaphambi kokuhlolwa;
- Ungenzi ukuhlolwa ngokushesha emva kokuya esikhathini, udinga ukulinda izinsuku ezingu-3-5 ngemuva kokuphela.
Ngaphambi kokuhlaziywa kwegazi, akukho ukulungiselela okukhethekile.
I-PCR - ukuhlaziywa kubonisa ngani?
Kuyaziwa ukuthi ukuhlaziywa kwe-PCR kubonisa ukutholakala kwezifo ezihlukahlukene zegciwane ne-bacteria. Le ndlela ibuye isebenze ekutholeni izifo ezingavamile, ezingapheliyo. Ukuhlaziywa kwe-STI ngokusebenzisa indlela ye-PCR kwenza kube lula ukuhlukanisa i-agent ye-pathogenic ngisho nokuba khona kwamangqamuzana angabodwa wegciwane namagciwane. Kumele uqaphele ukuthi yiziphi izilingo ze-PCR ezifakwe ebhodini lokutheleleka ngokocansi, oku:
- I-Chlamydia;
- i-mycoplasma ;
- ureaplasmas (i-parvum ne-urealiticum);
- i-agent causative ye-gonorrhea;
- izinhlobo ezahlukene ze-papillomavirus yabantu;
- I-Trichomonas;
- izinga lokugcina;
- Abafundi.
Ngezifo ezithathelwanayo ezithombeni zobulili, impahla ye-PCR iyinhlayiya evela emgodini wesibeletho somlomo wesibeletho, i-urethra ne-vaginal. Ukulungiselela ukukhulelwa kufanele kuhanjiswe ngomthwalo omkhulu. Uma uhlela ukukhulelwa, ukuhlaziywa kwe-PCR kuyadingeka uma kwenzeka kukhona izinsolo zezifo ezithathelwanayo ezivame kakhulu. Futhi uma kukhona ukutheleleka, kungcono ukuyeka ukukhulelwa. Kufanele kuqaphele ukuthi izivivinyo zokukhomba lezi zifo ezingenhla kumele zingadluliselwa kowesifazane kuphela, kodwa futhi kumuntu.
Futhi, indlela ye-PCR yembula ama-pathogens alandelayo:
- i-hepatitis B ne-C amagciwane;
- I-Mycobacterium isifo sofuba;
- I-HIV;
- amagciwane omndeni we-herpes, kuhlanganise negciwane le-Epstein-Barr ne-cytomegalovirus;
- Ukutheleleka kwe-Helicobacter pylori.
Ukuchazwa kwemiphumela
Ukumiswa kokuhlaziywa kwe-PCR akubanga imbangela. Ngokuvamile imiphumela yezingcingo ze-PCR ingatholakala kanje:
- Umphumela ongalungile usho ukuthi i-agent efunwayo esetshenziselwayo ayitholakalanga ekufundeni.
- Umphumela omuhle ubonisa ukuthi kukhona i-DNA noma i-RNA pathogen. Okusho ukuthi, ngokuqiniseka okukhulu kungathiwa yi-microorganism ekhonjisiwe eyimbangela yalesi sifo.
Kwamanye amacala, ukuzimisela okulinganiselwe kwamagciwane amakhulu kwenziwa. Lokhu kuyiqiniso ikakhulukazi ezifweni ezibangelwa ama-microorganisms anenzuzo. Njengoba lezi zingqikithi zibonisa imiphumela yazo emibi kuphela uma inani lidlulele. Futhi, ukuhlaziywa kwe-PCR okubalulekile kubalulekile ekukhethweni kwezinqubo zokwelapha kanye nenhloso yokulawula ukwelashwa kwezifo ezibangelwa igciwane lesandulela ngculaza kanye ne-virus ye-hepatitis.