Enye iminyaka engu-10-15 eyedlule, ukuvundiswa kwe-in vitro kwakubhekwa njengento ethile yezesayensi yeqiniso. Namuhla, amashumi ezinkulungwane zemibhangqwana athole ithuba lokuzwa injabulo yobazali kanye nobaba phakathi kobuchwepheshe be-ECO. Enye yezindlela zanamuhla eziphumelela kakhulu zokwelapha ukungabi nabantwana kungukuqothulwa kwe-IVF nge-ICSI.
Ukukhulelwa kwe-IKSI - obani nokuthi kungani
I-ICSI isho umjovo we-intracytoplasmic wesilisa. Ngaphandle kwegama elingabonakali likhona inqubo elula ekuboneni kuqala: i-sperm ifakwe ngqo eqenjini ngosizo lwezingqikithi ezikhethekile ze-microinstruments. Ngenxa yokungeniswa, inqubo ye-ICSI ibukeka sengathi igalelo. Futhi lokhu kuchaza ukuphumelela okuphezulu kwendlela: kudingekile isidoda esisodwa sekhwalithi, yonke imisebenzi eyenziwa yi-embryologist. I-sperm isehlala kuphela ukutshala iqanda, ukuhlangana ne-nuclei yakhe. Ngakho-ke, i-ICSI isetshenziselwa ukukhulelwa phambi kwezinhlobo ezimbi kunazo zonke zokuhlukunyezwa kwamadoda, ezingenakulungiswa ekwelapheni (isibonelo, ngokungabikho kokuzalwa komzimba okwenziwe nge-spermatic noma ukungabi khona kwe-spermatozoa evuthiwe ejaculate).
Ngaphezu kwalokho, ukukhulelwa kwe-ICSI kunqunywe kula macala alandelayo:
- izinkomba ezimbi zokubunjwa kwe-sperm;
- izinga eliphansi le-"thawed" le-spermatozoa;
- ukutholakala kwama-antibodies kumuntu.
I-ICSI kanjani?
Sizobona ukuthi i-ICSI iqhubeka kanjani. Okokuqala, ukuqothulwa kwe-ICSI ngokweqile kuyingxenye yohlelo lwe-IVF, okusho ukuthi zonke izinyathelo zokulungiselela - ukugqugquzela ama-ovarian, ukukhishwa, ukuqoqa nokwelashwa kwesidoda - kwenzeka ngokufanayo ngendlela ejwayelekile yokukhulelwa komzimba. Izingxabano ziqala esiteji sokulungiselela amaqanda ukukhulelwa: isisu se-embryolo sisusa izingqimba zakhe zokuzivikela ngosizo lwe-reagent ekhethekile. Ngaphansi kwe-microscope enamandla, i-sperm umhlabeleli nayo ikhethiwe. Zombili amangqamuzana abekwe emithonjeni ekhethekile lapho kudingeke khona ukushisa okudingekayo kanye nobuningi. Khona-ke iqanda lihlelwe nge-micropipette ekhethekile, i-spermatozoon isuswa umsila futhi ifakwe emkhathini. Ukusebenzisa abakwa-manipulators, ngokucophelela, ukulawula ukunyakaza ngalunye nokubona okwenzekayo kumakrofroscope, umbungu we-embryologist ufaka i-spermatozoon eqandeni. Inqubo ye-IVF IVF iphelile. Ihlala ilinde ukulinda ukukhulelwa kanye nokwahlukana kokuqala kweseli elisha.
ECO Statistics ICSI
Umphumela wokukhulelwa kwe-ICSI uphonywe yizici eziningi, okuyinhloko zazo eziyi-spermatozoa ne-ovules. Futhi amangqamuzana wesifazane awawutholi ngaso sonke isikhathi nge-hyperstimulation yama-ovari. Ezimweni ezingavamile, sebenzisa i-ICSI emjikelezweni wemvelo - ukuthola iqanda ngaphandle kwemithi. Kodwa-ke, lokhu kuyinkambinkimbi enzima kakhulu, okudinga ukuqeqeshwa okusezingeni udokotela futhi hhayi ukuphela njalo ngempumelelo.
Ngokwezibalo ze-ICSI, amathuba okukhulelwa ngokuphumelelayo ngemuva kwenqubo ye-ICSI ayidluli ku-60%. Lokhu kungenxa yokuthi ngesikhathi sokulungiselela nokuziphatha kwe-ICSI ovum
Noma kunjalo, ukukhulelwa kwe-ICSI akujwayelekile kakhulu kune-IVF ejwayelekile. Kunezizathu eziningana: imishini ebiza kakhulu ayitholakali kuyo yonke imitholampilo, ubunzima bezinqubo ngokwayo kanye nesitifiketi esiphakeme se-embryologist oqhuba ngayo.