Ukugcina isidoda

Le ndlela yokugcina isisu (i-ejaculate), njenge-cryopreservation, isanda kakhulu kwi-cryomedicine. Kuhlanganisa ukwengeza isikhala esikhethekile ku-fluid seminal, futhi ngokuqhubekayo ukuwuqothula ngokusetshenziswa kwamanzi omfutho we-nitrogen. Cabanga ngale ndlela yokulondoloza amangqamuzana egciwane lesilisa ngokuningiliziwe futhi akutshele ukuthi yisiphi isibindi esilungele ukukhuphula kanye nesitoreji esengeziwe.

I-cryopreservation ikwandisa kanjani amathuba okukhulelwa?

Ngomanyolo wemvelo, imfihlakalo yezinsipho zesilisa zobulili ixubene ngokuhlanganyela namaseli e-epidermis, ngenxa yalokho, ushintsho oluthile ku-pH yesikhulumi senzeke, okuholela ekubhujisweni kwe-lipoprotein cover of spermatozoa, i.e. ukuze kusebenze. Njengoba kulesi simo, ukuphila kwamangqamuzana okubeletha kunciphise kakhulu, okungabandakanyi ukusetshenziswa kwabo kwe-IVF. Yingakho indlela ye- cryopreservation isetshenziswa .

Le nqubo ikwandisa impilo yesikhala sembewu, futhi ivumela:

• kuncishiswe umsebenzi we-spermatozoa;
• ubahlinzeka ngomthombo owengeziwe wamandla;
• ivikela izitho zangasese zegciwane kusukela ekubhujisweni;
• kuvimbela ukuqoqwa kwemikhiqizo yezinto ezinobuthi;
• kuvimba ukukhiqizwa kwezincane ezincane ejaculate.

Yimaphi ama-mediums asetshenziselwa ukugcina isithombo?

Ukuze kwenziwe lula, inqubo ebizwa kanjalo yokuchoboza i-ejaculate, edingekile ukuhlukaniswa kwamagciwane e-germ, ukusuka ngaphambi kokuhlunga isidoda. Ama-reagents akhethekile asetshenzisiwe.

Kuze kube manje, kuyisiko ukusebenzisa imidiya yokwenza isilondolozi sembewu, ngayinye efuna izimo ezikhethekile. Ngokuvamile, lolu hlobo lwemvelo luqukethe izingxenye eziningana, ngokuvamile okungenani ezintathu. Ngakho-ke, ngesakhiwo sabo kunokwenzeka ukuba banike ushukela, ngokuvamile futhi asetshenziswe i-glucose ne-lactose, i-citrate ye-sodium.

Uma sikhuluma ngamakhemikhali athile angasetshenziswa njengemidiya yokugcina isilisa, khona-ke phakathi kwalezi zingabizwa ngokuthi i-Tris-buffer, i-Trilon B, i-EDTA, i-Spermosan PPK.

Ngaphansi kwaziphi izimo i-ejaculate igcinwe?

Ubuchwepheshe bokuqanda nokugcina isibhedlela kudinga ukubambelela embusweni okhethekile wokupholisa, kanye nokusetshenziswa kwamadivaysi akhethekile. Kulesi simo, igumbi lokubhubhisa ngokwalo kufanele lihlomele amadivaysi akhaza umoya.

Ngaphambi kokuba iqoqo le-ejaculate liqoqwe, wonke amadivaysi adingekayo ukuze kusetshenziswe i-cryopreservation, ikakhulukazi zonke izinhlobo ze-flasks, ama-cylinders aphethwe yisiqu, ama-pipettes, izihlungi zephepha zikhishwa kwikhabhinethi ekhethekile, ekushiseni kwama-130-150 degrees. Ngaphambi kwenqubo yesampula eqondile, zifakwa ku-thermostat ekhethekile, egcina ukushisa okuqhubekayo kwama-degrees angu-37.

Lapho isampula yesilisa sesilisa isuswa, ifakwa ebhokisini eliyinyumba. Ukushisa kokugcina kwesidakamizwa kufanele kube njalo. Inqubo yokupholisa yenziwa ngezigaba ezimbili.

Ekuqaleni kwawo, i-ejaculate ifakwa kuqala egumbini elibandayo, lapho izinga lokushisa liyancipha kancane kancane. Njengomthetho, inani lalo li--35 degrees. Emva kwalokhu, kukhishwa imfucuza ejulile, ukufaka ibhola elikhethekile nesidoda e-nitrogen egazini. Kulesi simo, impilo yesibalo sembewu ingafinyelela emashumini ambalwa eminyaka.

Ukuze usebenzise i-ejaculate, i-cryopreserved ngaphambilini, umkhumbi kanye nawo ufakwa emanzini afudumele, lapho kukhishwa khona kancane. Ngemuva kwalokho, ama-cryoprotectants asetshenzisiwe asuswa, ngokuphindaphindiwe okuphindwe kabusha kwesidoda ku-centrifuge. Ngemuva kwalokho, uketshezi lwamaminerali ngokwawo lubekwe endaweni ephakathi komsoco lapho i-spermatozoa ifakwa khona kamuva.